| Summary: | 碩士 === 國立臺灣科技大學 === 材料科學與工程系 === 102 === In this thesis, nanofibers of poly(lactic acid) (PLA) blended with chitosan (CS) were fabricated via the electrospinning from solutions containing CS and PLA in various ratios. In particular, the solvent of the solution was a mixture of chloroform, ethanol, acetic acid and water. The morphological characteristics of nanofibers was examined using scanning electron microscope (SEM). The cytotoxicity of nanofibers was evaluated based on the proliferation of fibroblasts. The presence of chitosan in CS-PLA nanofibers were characterized using UV-Visible spectrophotometer. The antibacterial activity of these CS-PLA nanofibers was determined against Escherichia coli (E. coli).
SEM images showed that average diameter of Chitosan-PLA nanofibers were nanometer length of scale. In addition, the number of beads increased with the increase of chitosan content in the Chitosan-PLA nanofibers. The uptake of an acidic dye (orange II sodium salt) indicated the presence of chitosan in the nanofibers. By culturing with L929 fibroblasts, CS-PLA nanofibers showed good cell viability, indicating non-cytotoxicity. Furthermore, CS-PLA nanofibers exhibited antibacterial activity against E. coli.
The high antibacterial activity and biocompatibility suggests that these CS-PLA nanofibers have a potential in biomedical fields, especially for wound dressing applications.
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