Layer-by-layer deposition of amine and carboxylic functionalities by plasma polymerization and to investigate the potential bioapplications

• Main Authors: Yi-ren Su, 蘇義仁
• Other Authors: Meng-jiy Wang
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/68409323174918412503

碩士 === 國立臺灣科技大學 === 化學工程系 === 102 === This thesis proposed the layer-by-layer plasma polymerization method to prepare multilayered thin films containing amine and carboxylic functionalities. The parameters for plasma polymerization for the deposition of aniline and acrylic acid precursors were investigated physical-chemically on the applied plasma power, deposition time, and the orders of precursor for deposition. Moreover, the deposited multilayered thin films were characterized in terms of film thickness, surface wettability, surface functionalities, and chemical compositions. For the applications of the prepared multilayered thin films, L-929 mouse fibroblasts were directly cultivated on the samples to evaluate the biocompatibility. The results of LDH (lactate dehydrogenase) assay showed that the surfaces prepared by plasma polymerization with different top layer of functionalities revealed distinct responses to cell growth. Among all the samples, the two and four layered thin films, N69/A271 and (N69/A271)2, revealed the most improved cell density that 101 % and 116 % cell density were obtained when compared with the pristine surface. Moreover, the confocal microscopy images also clearly showed that the well extended cell morphology on N69/A271 and (N69/A271)2. In order to prove the applicability of the proposed method for preparing multilayered thin films, the same plasma polymerization parameters were applied on polystyrene for preparing multilayered thin films and the results confirmed that the four-layered multilayered thin films showed higher cell density in comparison with the pristine polystyrene. The overall results indicated that the proposed layer-by-layer plasma polymerization for preparing multifunctional thin films allow to promote biocompatibility and could be successfully applied different substrates for applications in biomaterials.