| Summary: | 碩士 === 國立臺灣科技大學 === 化學工程系 === 102 === In this thesis, poly(2-ethyl-2-oxazoline) (PEOXA) was synthesized by cationic ring opening polymerization. The synthesized PEOXA was further used to be altered with different end functional groups in order to be incorporated on polymethyl methacrylate (PMMA) and stainless steel (SS) surfaces by plasma induced grafting and cycloaddition reaction methods, respectively. The synthesized PEOXA molecules were evaluated by Fourier Transform Infrared Spectroscopy (FTIR) and 1H NMR. The effects of surface modifications on surface of PMMA and stainless steel were characterized by ATR-FTIR, water contact angle (WCA), and Electron Spectroscopy for Chemical Analysis (ESCA). The surface morphology was evaluated by scanning electron microscopy (SEM). The cell adhesion was evaluated by directly cultivating L-929 fibroblasts on the samples.
For the first part of this thesis, PEOXA was altered to possess alkene end groups to form PEOXA¬C=C. Two different number average molecular weight (Mn), 1790 and 2603, of PEOXA-C=C were synthesized and were firstly immobilized on plasma pretreated PMMA and stainless steel, which was followed by Ar plasma induced grafting. The outcome of PEOXA¬C=C grafting was clearly demonstrated by the change of water contact angle from 80° (PMMA) and 52.7° (SS) to 60°, on both substrates. Moreover, the ATR-FTIR results revealed that a C-C-N peak appeared at 1627 cm-1, confirming the incorporation of PEOXA moieties on substrates. The efficiency for resisting cell adhesion of L-929 fibroblasts reached up to 55 % for 0.15 M of PEOXA¬C=C polymer grafted on PMMA and stainless steel surfaces.
For the second part of the thesis, the end groups of PEOXA was altered to be alkyne to form PEOXA¬C≡C. Two different molecular weight of PEOXA¬C≡C, 1548 and 4262, were synthesized. In addition, the azide functional groups were incorporated on stainless steel by silanization approach. Then the two different molecular weight of PEOXA¬C≡C were immobilized on stainless steel with azide groups which were further underdgone the azide-alkyne cycloaddition reactions. The incorporation of PEOXA¬¬—C≡C on stainless steel resulted in change of water contact angle from 52.7° to 60°. Moreover, the functionalities were also verified by ATR-FTIR. The N-C=O and C-N bonds were detected after PEOXA¬¬—C≡C incorporated on stainless steel surfaces according to the results of ESCA analyses. The efficiency of anti-cell adhesion was up to 63 % by using 11 mM of PEOXA¬¬—C≡C with Mn of 4262 on stainless steel surface.
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