| Summary: | 碩士 === 國立臺灣科技大學 === 化學工程系 === 102 === Electroporation is a technique which applies high pulse voltage to generate pores on the cell membrane of bacteria. Consequently, some of intracellular compounds will be released out of the cell after electroporation that leads the cell to die. This thesis focused on using silver nanowires (AgNWs) which have high conductivity and a large number of nanoscale tips to generate high electric field strength at low applied voltage. AgNWs were coated on a very porous electrode made of carbonized gauze so that bacterial suspension can easily pass through. The bacteria killing effect of electroporation was studied by flowing Eschericia coli suspension in deionized water through the electrodes at different rate, voltage, bacterial concentration, electrode layers, and etc. The best bacteria killing ability was observed by applying 20V with 3.95 ml/min of flow rate for 20 min. Under the same condition, by increasing electrode layers to 4 layers, the bacteria killing ability enhanced and sustained to another 20 min. The different water sources have a strong effect on bacteria killing efficiency by electroporation. The AgNWs-carbonized gauze shows the similar bacteria killing ability for the bacteria in phosphate buffer solution, in pond water as well as in deionized water. However, bacteria in LB medium couldn’t be killed effectively due to the rich nutrient content that can provide bacteria to grow after damage.
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