Highly Selective Biomolecule-Cellulose Complexes for Palladium-Polluted Water Remediation and Its Potential Application for Degradation of Trichloroethylene

• Main Authors: Ian Sofian Yunus, 謝源生
• Other Authors: Shen-long Tsai
• Format: Others
• Language: en_US
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/73474810606232793181

碩士 === 國立臺灣科技大學 === 化學工程系 === 102 === This work examines the adsorption of Pd(II) and other interfering ions in natural waters on PdBP-CBD-cellulose complexes and its conversion to a bioinorganic catalyst for dechlorination of trichloroethylene (TCE). In-frame fusion protein, which is composed of palladium binding peptides (PdBP) and cellulose binding domains (CBD), was expressed in Escherichia coli (E. coli) and allowed to bind cellulose for remediation of palladium-polluted water. The adsorption of Pd(II) was best at pH 3.08. The results indicated the wide working pH condition of PdBP-CBD-cellulose complexes and the insignificant effect of temperature on palladium adsorption. Based on the Langmuir adsorption isotherm, it was found that the maximum adsorption capacity of the PdBP-CBD-cellulose was 169.49 mg.g-1 displaying a high adsorption capacity toward Pd(II). The Langmuir adsorption isotherm was applicable to describe the monolayer coverage adsorption processes. Kinetics of the Pd(II) removal was found to follow pseudo-second order rate equation. The results also showed that PdBP-CBD-cellulose omplexes exhibited a good reusability for Pd(II) adsorption. Furthermore, in the presence of Pt(IV), the PdBP-CBD-cellulose complexes still showed highly selective adsorption of palladium ions allowing efficient recovery of Pd(II). These results are important for developing and optimizing the selective removal of different metal ions in mixed solutions by biomolecules-cellulose complexes. In addition, the biorecovered Pd(II) was further used as catalyst for dechlorination of trichloroethylene (TCE). Complete dehalogenation of 146 mg.L-1 TCE was achieved within 20 min using approximately 0.14 mg nanopalladium catalyst. This study demonstrated the possibility of environmentally-friendly production of palladium catalyst and its application for TCE degradation.