Effect of EGCG and Fluoride against Soft-drink-induced Dental Erosion: an in vitro study

• Main Authors: Yu-Chen Lu, 盧育成
• Other Authors: Chun-Pin Lin
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/dwusua

碩士 === 國立臺灣大學 === 臨床牙醫學研究所 === 102 === Objectives 　　Dental erosion is a rising problem recently, and the frequently soft-drink consuming nowadays might be one of the reasonable attributions. The present study is aim to evaluate the effect of fluoride and EGCG against soft-drink-induced dental erosion in vitro. Materials and methods 　　A soft drink is used as acid source of erosion in the present study. 40 enamel samples and 40 dentin samples, which were made of human extracted teeth, were distributed into 4 group and received pre-treatment by deionized water (DW), 0.5M NaF (F), 400μM EGCG (EGCG), and combination of 0.5M NaF and 400μM EGCG (Mix) in rinse for 4 minutes respectively. Afterwards, the samples were immersed in the soft drink for 15 minutes for 4 times with 60 minutes interval in artificial saliva. Then, the samples were analyzed of the change of average height, surface area changing, surface hardness and surface structure after erosion by confocal laser scanning microscope, Vickers’s hardness tester, and scanning electronic microscope. Results 　　In enamel samples, the F group showed higher surface hardness in contrast to the DW group, but it made no difference in surface area change and average height. The EGCG group and the Mix group, in contrast to the DW group, showed higher surface hardness, less surface area change and lower average height. Besides, the EGCG group exhibited highest surface hardness of 4 groups. 　　In dentin samples, compared to the DW group, the F group showed higher surface hardness and lower average height. In the other hand, the EGCG group and the Mix group exhibited higher surface compared to the DW group. As for surface area change, the outcomes were similar in all groups. Conclusion 　　The present study demonstrated that all of 0.5M NaF, 400μM EGCG, and combination of both rinse could reduce the amount of soft-drink-induced dental erosion in vitro. EGCG demonstrated anti-enamel-erosion effect and fluoride showed anti-dentin-erosion effect. The inhibitory effect of Mix rinse was neither simply combination effect nor the best of all. There might be a interaction or competitive effect between NaF and EGCG, and it needs further studies to assure.