Characterization of Activin/TGF-β Signaling in the Wound Healing and Early Anterior Regeneration of Aeolosoma viride

• Main Authors: Fen-Han Hou, 侯棻涵
• Other Authors: Jiun-Hong Chen
• Format: Others
• Language: en_US
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/zcyhv4

碩士 === 國立臺灣大學 === 生命科學系 === 102 === Activin, a TGFβ superfamily protein, is known to regulate cell proliferation, inflammation, apoptosis, regeneration, and wound healing. Previous researches demonstrated that activin is necessary for wound healing in mice and fin regeneration in zebrafish. Recently, follistatin has been linked to regeneration in planarians. In this research, we investigated the roles of activin in the wound healing of a small fresh water annelid, Aeolosoma viride. A.viride can fully regenerate the head within 5 days after amputation. The results showed activin expression is upregulated during regeneration and activin receptor is densely expressed at the wound site and the blastema. Furthermore, A. viride treated with a chemical inhibitor of activin/TGF-β, SB 505124, obviously inhibited wound healing and impaired regeneration. Therefore, we infer that activin/TGF-β signaling might mediate wound healing to affect anterior regeneration in A. viride.