| Summary: | 碩士 === 國立臺灣大學 === 園藝暨景觀學系 === 102 === Banana (Musa sapeintum L.) is a perennial herbaceous plant of the family Musaceae and genus Musa, and it is an important cash crop in Taiwan. Banana peel, which accounts for about 40% of the total weight of banana fruit, is the by-product of the banana processing and agricultural industry. The peels are often used as feed and fertilizer. Previous studies have shown that banana peel is rich in functional compounds. However, due to its high nitrogen and phosphorus content, and high water content, this by-product is becoming a burden to environment. In this study, banana peel and frozen banana peel were used as materials for water and methanol extraction, which include concentration at 40° C or 70° C, to obtain six different extracts. The colorimetric detection of Dopachrome producing capacity is use to evaluate the effect of tyrosinase activity of six samples. The results showed that banana peel methanol extract with concentration 8 mg/mL which concentrated at 40°C displayed 69.75% higher in the dopachrome production. Banana peel methanol extract with concentration 8 mg/mL which concentrated at 70°C displayed 9.15% higher in the dopachrome production. Frozen banana peel methanol extract with concentration 8 mg/mL which concentrated at 40°C reduced 24.36% of dopachrome production, and with the same concentration which concentrate at 70°C reduced 48.41% dopachrome production of the same concentration. Therefore, frozen banana peel is found the inhibitory activity to tyrosinase, and it is uncompetitive inhibitor to tyrosinase. After liquid-liquid partition by organic solvents, butanol fraction of banana peel methanol extract concentrated at 40°C has 79.15% higher in dopachrome production, and butanol fraction of frozen banana peel methanol extract concentrated at 40°C reduced 53.74% dopachrome production. The compounds in the butanol fraction of frozen banana peel methanol extracts concentrated at 40°C may cause changes in the production of dopachrome, which need to be further identified.
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