| Summary: | 碩士 === 國立臺灣大學 === 植物病理與微生物學研究所 === 102 === A large number and wide variety of microorganisms are associated with plant roots and the genetic elements of rhizosphere microbiome determine the plant health. Bacillus cereus C1L screened from the rhizosphere of Lilium formosanum could reduce severe symptom development of southern corn leaf blight (SCLB) caused by Cochliobolus heterostrophus. In this research, the changes of maize rhizobacterial community post C1L application and C. heterostrophus infection were analyzed by metagenomic mining. The abundance of Pseudomonas rhizobaceria remarkably increased at 3 days post C1L application, then decreased to the original state at 6 days after C1L application. Moreover, the abundance of Pseudomonas rhizobacteria was higher at 3 days post inoculation with C. heterostrophus on maize plants 3 days post C1L application as compared with that without C1L pretreatment. These results indicated a high relatedness between Pseudomonas and disease resistance state of maize. Besides, the relative abundance of Enterobacter, Pseudomonas, Paenibacillus and Bacillus rhizobacteria increased in maize rhizosphere 3 days post inoculation with C. heterostrophus as compared with that in the control. On the other hand, 12 isolates of Pseudomonas and 14 isolates of Bacillus, reducing at least 30% disease severity of leaf blight in corn seedlings, were screened from maize rhizobacterial community. These bacterial isolates capable of enhancing plant disease resistance were presumed and have potential for further development of application.
|
|---|
