Dietary supplementations of DHA and Goji improve fatty liver hemorrhagic syndrome in laying hens

• Main Authors: Shih-Yu Fan, 范詩宇
• Other Authors: Shih-Tong Ding
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/95107365795515724439

碩士 === 國立臺灣大學 === 動物科學技術學研究所 === 102 === Fatty liver hemorrhagic syndrome (FLHS) is a metabolic diease of laying hens. It is characterized by the accumulation of excess fat in the liver due to enhanced lipogenesis, leading to rupture and hemorrhagic lesions with liver of hens during late laying stage. It tends to result in sudden egg production drop and increase mortality in some serious cases and thus causes considerable economic loss by the egg producers. In this study, we aim to improve FLHS by dietary supplementation of docosahexaenoic acid (DHA) and Goji in laying hens, and furthermore to produce functional eggs in order to increase the value of eggs as well as. Eighty-four Hy-line strain W-36 layers (36 week of age) were used in the study with a factorial design of dietary supplementation of Algal DHA oil and Goji (Algal DHA oil 0, 1% × Goji 0, 2%). Feed and water were supplied ad libitum during the trial for 24 weeks (36-60 week of age). Results showed that Algal DHA oil and Goji had no effect on the body weight and egg production (P > 0.05). In all treatments, no significant difference in plasma total cholesterol (TC) and triglyceride (TG) level were observed (P > 0.05). Compared to the control diet (Algal DHA oil 0%, Goji 0%), Algal DHA oil treatments decreased hemorrhagic score, TC and TG content and fat accumulation in the liver (P < 0.05) at 60 week of age. Goji treatments reduced hepatic TC (P < 0.05) at 60 week of age. However, there were no significant difference in all treatments along age at 36 and 48 week (P > 0.05). Moreover, Algal DHA oil suppressed hepatic transcript level of lipogenic enzymes, including acyl-CoA oxidase (ACC) and fatty acid synthase (FAS) from 48 to 60 week of age (P < 0.05), consistently with attenuated fat accumulation in the liver. All treatments had no significant effect on the transcript abundance of proinflammatory cytokine genes, such as tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6). In addition, yolk color from hens with dietary supplementation of Goji had higher a (red) and b (yellow) values than those from control treatment. In conclusion, Algal DHA oil and Goji may have the potential to be used as a dietary supplementation to improve FLHS in laying hens, and also increase the economic and nutritional value of the eggs.