Effect of Narrow Bandwidth Infrared Radiation on the Cervical Cancer Cells Hela Expressions

• Main Authors: Shin-Chi Yeh, 葉欣綺
• Other Authors: SC Lee
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/15124620761272236167

碩士 === 國立臺灣大學 === 生醫電子與資訊學研究所 === 102 === The purpose of this research is to study the infrared irradiation effect on the cervical cancer cells Hela growth and migration. The plasmonic thermal emitter with broad bandwidth (3~3.8 μm) and narrow emission waveband (3.8, 4.4, 4.8 μm) were designed and used to illuminate the cells for 48 hours to investigate the specific wavelength that affects the cell expression most significantly. Infrared emitters used in this study are fabricated based on the theory of surface plasmon. The infrared source can be achieved by heating the triple layer structure which consists of a SiO2 layer sandwitched between two Ag films on the silicon substrate, it is called metal/dielectric/metal (MIM) structure. The top Ag layer is perforated by periodic hole array, and the emission wavelength can be altered and selected by changing the lattice constant and diameter of the hole arrays. It is found that the growth of Hela cell responds the most after illumination by PTE with peak wavelength at 3~3.8 μm and 3.8 μm, the growth of Hela cell was inhibited significantly. Besides, IR may regulate mitochondrial membrane potential, activate the downstream of apoptosis to decrease cell viability. And it also showed that 3~3.8 μm and 3.8 μm decreased the cell movement mostly after 48h IR exposure. In general, after infrared illumination under specific wavelengths, hela cells can be diminished in vitro. The study implies that IR illumination can prohibit the cell growth and promote cell apoptosis.