Fabrication of Zwitterionic Porous Hydrogels for Endothelial Cell and Tissue Engineering

• Main Authors: Chih-Yeh Lin, 林志燁
• Other Authors: Jiashing Yu
• Format: Others
• Language: en_US
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/34135814215531321929

碩士 === 國立臺灣大學 === 化學工程學研究所 === 102 === Zwitterionic hydrogels have been investigated for a number of applications in tissue engineering. The most important characteristics include the non-fouling property and it can be highly hydrated. So, with some specific structural modification, it is an appropriate material of tissue engineering scaffold. In this study, we first introduced the generation of 2-D poly-(SBMA) hydrogels surfaces. By incorporating with RGD and QK, endothelial cells can attach to the surface well to and proliferate in a short-term culturing. However, the mechanical property, which plays a crucial role directing the cellular functions and supporting the structures, decreases when peptides graft onto hydrogels. Manipulating the mechanical property was thus necessary and the most related factor was the monomer concentration. From our results, the higher amount of SBMA caused greater stiffness in hydrogels. In the second part of our study, we fabricated 3-D porous hydrogels for cell scaffolds by some novel methods. The salt/particle leaching method is more reliable than gas-foaming method to fabricate homogeneous and open-interconnected pores within the hydrogel. Using a salt/particle leaching method, we can control the pore size before leaching. Endothelial cells within scaffolds were also investigated the morphology by SEM and histological analysis was conducted in vitro and in vivo.