De-emulsification of oil-in-water emulsion by Rhodococcus erythropolis NTU-1 bioflocculi grown in diesel

碩士 === 國立臺灣大學 === 化學工程學研究所 === 102 === NTU-1, a highly efficient demulsifying strain, was isolated from petroleum contaminated soil and identified as Rhodococcus erythropolis by 16S rDNA gene analysis. NTU-1 powder with high demulsifying performance could be obtained by heat-drying after collecting...

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Bibliographic Details
Main Authors: Yueh-Ting Lai, 賴岳廷
Other Authors: 劉懷勝
Format: Others
Language:zh-TW
Published: 2014
Online Access:http://ndltd.ncl.edu.tw/handle/23854500095650265277
Description
Summary:碩士 === 國立臺灣大學 === 化學工程學研究所 === 102 === NTU-1, a highly efficient demulsifying strain, was isolated from petroleum contaminated soil and identified as Rhodococcus erythropolis by 16S rDNA gene analysis. NTU-1 powder with high demulsifying performance could be obtained by heat-drying after collecting bioflocculi in medium. This study first evaluated the demulsifying performance of NTU-1 cultured by diesel and n-hexadecane, and found that NTU-1 powder by diesel is a better choice for its lower cost and better performance. At an optimal quantity of dried NTU-1 powder for de-emulsification that was proportional to the volume of Tween-80 to the 2/3 power, a recovery of 95 % oil was achieved in 480 min. This was made possible when the oil/water/Tween80 volume ratio was set at 4/1/0.01. The higher O/W ratio and the lower viscosity both contributed to such a high oil recovery rate. The positive correlation between CSH (MATH) and oil recovery ratio also implies that the diesel grown NTU-1 culture may provide a commercial scale-up method to break oil -in-water emulsion directly at the primary oil recovery site.