The identification and control strategies development of the disease on the betel cause by opportunistic infecting soil bacteria in Taitung

• Main Authors: Jun-Cheng Lin, 林俊成
• Other Authors: Hsiang-En Huang
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/25463965658809777264

碩士 === 國立臺東大學 === 生命科學系碩士班 === 102 === Piper betle is a high production crop with value of about 4-8 billions annually. Due to high rainfall and humidity weather in Taiwan, diseases such as anthracnose, phytophthora disease, southern blight, powdery mildew and angular spot disease are highly-rated and common. In addition to these diseases, there are few microbe that can cause symptom in uncertain condition, name as opportunist pathogens that in Piper betle is unclearly. Thus the main purpose of this study is to identifity and control of the disease on the Piper betle cause by opportunistic infecting soil bacteria in Taitung. The result shown that disease index of Piper betle in the period of January 2011 to February was significantly higher than in other months of 2010. There are 4 independent opportunistic pathogens have been identified in this investigation, such as Acinetobacter baumannii (Xan3-L1S1), Cellulosimicrobium cellulans (Q1-L1S2), Sphingomonassp. (3-2a-L2S1) and unknown (J3-L2S2) that were unable to hydrolysis tween activity. PCR amplification with estA primer from DNA template of Xan3-L1S1 and J3-L2S2 got amplificated in the predict size. The isolated Xan3-L1S1 caused serious disease symptoms in young leaves and stems, but it did not in matured leaves. The isolated Q1-L1S2 caused serious symptoms in the young leaves, but did not in the stem. By contrast, isolated 3-2a-L2S1 only caused serious symptoms in the stem, but did not in the leave. The isolated J3-L2S2 caused minute symptoms in both young leaves and stems. Xan3-L1S1 could survive under heat stress environment at 40