| Summary: | 碩士 === 國立臺灣海洋大學 === 機械與機電工程學系 === 102 === This study adopted the microfluidic chip integrated with the phosphorescence phase
difference optical detecting system to develop the device for detecting local distribution of blood and
oxygen in the body of juvenile fish of single zebra fish. The microfluidic detecting platform combined
with two components: the pneumatic actuator positioning zebra fish microfluidic chip and the
phosphorescence phase difference optical inspection system. The pneumatic actuator positioning zebra
fish microfluidic chip was used to localize zebra fish in the flow channel to culture for a long time, and
to change external environment by changing injected fluid, or to immerse fish in agents for a long time,
and to carry off effluent via peristaltic pump, thus maintaining a stable stimulation of agents and
environment. Role of pneumatic actuator positioning zebra fish device: pneumatic deformation of upper
flow channel allowed fish to be localized only in wider flow channel than its body width. Each organ or
tissue can be measured for a long time under the stable localization. Phosphorescence phase difference
optical inspection system was used to control the light source projection figure of Blue-Laser through
digital micromirror device (DMD) within projector and to stimulate blood oxygen value in blood in the
area for detection, and then achieved the automatic real-time effects of stimulated measurement.we used
Oxyphors G4, Pd-meso-tetra-(3,5-dicarboxyphenyl(referred as Oxyphors G4 below) as the
phosphorescence sensing measurement index within juvenile fish of zebra fish. The index was injected
into fish bodies which were grown-up for 48h. In comparison of common phosphate buffered solution
(PBS) to HEPES as hydrogen ion concentration buffer in water flea injection, HEPES can maintain the
PH in 7.2-7.4 which is near the PH value within fish body for a long time to lowering motality. 9.2nl of
HEPES with lower lethality was injected in a concentration of 200uM to establish the sensing system in
vivo.
The experimental findings founded that : the ratio of blood and oxygen in fish body was decreased
gradually to 0% within 10 minutes in the low oxygen environment (0.01 mg/L O2); however, the ratio
of blood and oxygen of some fishes were recovered to the ratio within the general water body under the
recovered oxygen environment(7-8 mg/L O2). We measured the changes in blood and oxygen within two
different blood pooling areas in zebra fish body and detected the difference of the blood and oxygen
variability in external extreme environment between the loop valley and caudal veins, and the initial
value of ratio of blood and oxygen in veins was lower, also the blood oxygen level was decreased quickly
to 0%.
This study can establish a measurement way for the detection mechanism in the body of living organisms.
It is expected that this blood oxygen sensing system can be applied to the micro-tumors model of animals
like zebra fish in future to observe the correlation between active state of cancer cells to the blood and
oxygen
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