An Investigation on the Hemocompatibility and Adhesion of Heparin/Collagen Polyelectrolyte Multilayers Coated on Titanium Substrate by Layer-by-Layer Technique – Dopamine as the Interlayer between Coatings and Titanium Substrates

• Main Authors: Lin, Hou-Cheng, 林厚丞
• Other Authors: Chou, Chau-Chang
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/88443753775045369875

碩士 === 國立臺灣海洋大學 === 機械與機電工程學系 === 102 === This thesis used layer by layer technology to coat heparin/collagen multilayer on titanium substrates. Dopamine as the interlayer between coatings and titanium substrates was applied and the coating’s hemocompatibility and adhesion was investigated. Two different pretreatments on sample. One was putting samples into 5M NaOH solution 60℃for 24 hours another was putting samples into 2 mg/ml dopamine solution for 8 hours. After finish the pretreatment that we took samples immersed in poly-L-lysine solution for 30 minutes to obtain positive ion. And next step was repeated exchange heparin and collagen solution to coating anticoagulant film on titanium substrates until the coatings to the desired number of layers. The surface topography, chemical composition and hydrophilicity of the films were investigated by scanning electron microscopy, Fourier transform infrared spectroscopy, and water contact angle measurement. The study of the adhesion of the multilayers was conducted by nano-scratch test. The hemocompatibility was evaluated by measuring the hemolysis ratio, platelet covered area and activated-partial-thromboplastin-time (APTT) in vitro. The results indicated that two kinds of anticoagulation multilayers was successfully coated on titanium substrates. The use of dopamine as the interlayer between coatings and titanium substrates is beneficial to enhance the hemocompatibility and adhesion as well.