| Summary: | 碩士 === 國立臺灣海洋大學 === 水產養殖學系 === 102 === In this study, intends to investigate the effect of preservation and efficacy of multiemulsified oral inactivated vaccine against grouper iridovirus. Firstly experimental inactivated Grouper iridovirus (GIV) vaccines were prepared, after safety testing, stored at 4℃ and 25℃ room temperature for 8 months and 12 months, multie-mulsified vaccine are not occurring disintegrations. Further compared the efficacy with the new preparation vaccine protective, we had mix these five vaccines into fish feed and fed once every other week. Challenge with GIV at day 10 and day 20 post vaccination. The relative survival rate of the new preparation vaccine are 60% and 50%, of the vaccine preserved at 4 ℃ and 25 ℃ for 8 month are around 40 %, and of 4 ℃ and 25 ℃ for 12 month are decreased to 20%. Analyzed the anti GIV antibody titre in serum from each groups at day 1, 4, 7 post vaccination, the results which had compared with non-vaccinated control group indicated that antibody had stimulated in all the vaccinated fish. The antibody titre had slightly decreased at day 10, 14 post vaccination were observed in all the groups, especially the group fed with vaccine had preserved at 4 ℃ and 25 ℃ for 12 month. Finally, we analyzed the gene expression of MHC-I, TNF-α, IgM, Mx and INF-γ in spleen and head kidney. The results suggest that vaccinated groups were much higher than non-vaccinated groups, such as INF-γ in spleen. Mx gene expression has significant difference between new prepared vaccine and other preserved vaccines. All the results had suggested that the inactivated vaccine could be well preserved using multie- mulsified preperation, and still could stimulate immune response after storing at 4℃ for 8 month.
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