| Summary: | 碩士 === 國立臺灣海洋大學 === 水產養殖學系 === 102 === In aquaculture, CBM (Complex Beneficial Microorganisms) can be used in different ways, such as water processing, disease prevention, feed potentiation, and nutrient regulation. The objective of present study is using CBM in aquaculture wastewater improvement and development of biological feed. To accomplish this objective, several specific aims were established；1) CBM was embedded as immobilized biocatalyst (IBC) to improve aquaculture water quality; 2) optimized condition for use functional CBM in ASSF (Anaerobic solid state fermentation) for soybean meal to increase small peptide content, phytic acid and crude fiber can be reduced; 3) using the mixture of functional CBM fermented soybean meal with marine protein concentrate for fish meal substitution. In present study, immobilization pellet were made by taking polyvinyl alcohol (PVA) and sodium alginate (SA) as the skeleton carrier, using embedding method to embed CBM. The results of present study, that CBM (IBC) was used in simulated wastewater of freshwater and wastewater, it brought 、fish tank treatment can effective reduce ammonia, nitrite, COD. In the most detail optimum fermentation condition were( wheat bran /soybean meal ratio) (w/w) 11.11%, solid-liquid ratio (v/w) (1: 0.6444) and (Inoculation/Soybean meal ratio) (v/w) 9.33% in 36℃, 7days. Under the optimum function proces level determined，the content of TCA-NSI reached 14.89％. Soybeam meal after TBFCBM (Functional CBM contain Trichoderma and Bacillus licheniformis) fermentation the content of phytic acid decreased 63% and crude fiber decreased 22.8%. In addition, in a growth performance test, there was no significant difference (P >0.05) in weight gain of orange-spotted grouper(Epinephelus coioides) was found by using 30% fish meal substitution with the mixture of functional CBM fermented soybean meal and marine protein concentrate.
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