| Summary: | 碩士 === 國立臺灣師範大學 === 人類發展與家庭學系 === 102 === Obesity is a chronic metabolic disease resulting from an imbalance between energy intake and energy output. It is caused by the interaction of multiple genetic and environmental factors. Health hazards associated with obesity are serious and include heart disease, sleep apnea, diabetes, and cancer. Thus, the development of agents that may offer safer and more effective alternatives for weight management is needed.
Antrodia cinnamomea, known as “niu-chang-chih”, has been traditionally used for the treatment of food and drug intoxication, diarrhea, abdominal pain, hypertension, and liver cancer. However, little is known about anti-obesity potential of A. cinnamomea. In this study, we evaluate the anti-adipogenic and anti-obesity activities of extracts of A. cinnamomea cultured on artificial agar plates.
The aqueous and ethanolic extracts were prepared form dried fruiting bodies of A. cinnamomea. There are 10 triterpenoids found in ethanolic extracts of A. cinnamomea, including antcin K, 4,7-dimethoxy-5-methyl-1,3-benzodioxole, antcin C, zhankuic acid C, dehydrosulphurenic acid, zhankuic acid A, zhankuic acid B, 15-α-acetyl-dehydrosulphurenic acid, dehydroeburicoic acid, and eburicoic acid. The results showed that a low molecular weight polysaccharide (less than 14kDa) was predominantly present in the polysaccharide fractions of A. camphorata. In addition, galactose and fucose were major neutral sugars in polysaccharide fractions of A. cinnamomea.
We investigated effects of ethanolic (ACE) and aqueous extract (ACW) of A. cinnamomea on adipogenesis of murine 3T3-L1 cells at different differentiation stages. Our results showed that both extracts inhibited lipid deposits. ACW, polysaccharides (PS) and non-polysaccharides fractios (NPS) of ACW exhibited significant anti-adipogenic effect. The anti-adipogenic function of ACW is through inhibition of mitotic clonal expansion in the early phase of adipogenesis. During adipocyte differentiation period, ACW and NPS significantly decreased key adipocyte differentiation-associated markers, PPARγ, C/EBPβ, C/EBPα, FAS, and aP2 expression. PS suppressed DMI-induced ERK phosphoylation and mRNA expressions of PPARγ and aP2.
ACW was assayed for alleviative effects on obesity. An obesity animal model was established in 5-wk-old C57BL/6J male mice fed with high-fat diet (HFD) for 12 weeks. The HFD mice exhibited significant body weight gain and impaired glucose metabolism. Mice with ACW co-administration (HFD+ACW group) showed significantly lower serum insulin, leptin and HOMA-IR value and ameliorated liver damage (AST values and hepatic cholesterol and triglyceride levels). HF+ACW group had significantly lower body weight gain due to decrease HFD-induced visceral fat accumulation (perirenal adipose tissue, and mesenteric adipose tissue).
In conclusion, A. cinnamomea aqueous extract and its fractions had anti-adipogenic effect in vitro. Furthermore, A. cinnamomea aqueous extract showed the anti-obesity potential in vivo. Our results suggested that A. cinnamomea extract may be applied to body weight control in future.
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