| Summary: | 碩士 === 國立臺灣師範大學 === 人類發展與家庭學系 === 102 === Acne vulgaris is a common skin disease involving pilosebaceous follicle. The pathogenesis of acne vulgaris is multifactorial, including increased sebum production, comedogenesis and Propionibacterium acnes proliferation. P. acnes plays an important role not only in the process of inflammation but also in the formation of comedones. Previous studies have shown that P. acnes is one of the most important factors in acne. P. acnes activate toll-like receptor-2 on monocytes, which triggers the release of proinflammatory mediators（TNF-, IL-8）, some studies have found that reactive oxygen species (ROS) will participate in the progression of acne. Medium chain fatty acid（MCFA）including caproic acid （C6:0）, caprylic acid （C8:0）, capric acid （C10:0）和lauric acid （C12:0）.
We investigated the effect of MCFA on inflammation caused by P. acnes.Human monocytes THP-1 cells and SZ95 were treated with P. acnes alone or in the presence of MCFA.Our previous studies have shown capric acid, lauric acid reduced P. acnes would activate monocytes to secrete pro-inflammatory cytokines.In addition, capric acid, lauric acid reduced P. acnes-induced inflammation of mice ear and then showed in vivo anti-inflammatory activity.
Our results showed capric acid effectively inhibited the growth of P. acnes. Capric acid, caprylic acid and caproic acid potently suppressed the production of pro-inflammatory cytokines such as tumor necrosis factor-, interleukin (IL)-8, and IL-1B by human monocytes THP-1 cells stimulated with P. acnes.
The IC50 of capric acid was 22.9 M, we further evaluated the anti-inflammatory mechanism of capric acid, the results showed that significantly inhibited P. acnes-enhanced phosphorylation of p38, ERK, JNK and p65.MCFA reduced P. acnes-induced inflammation of mice ear and showed in vivo anti-inflammatory activity.
Arachidonic acid（AA） significantly enhanced IL-8 by P. acnes-stimulated THP-1 cells, and AA stimulated lipogenesis of SZ95 sebocyte. Capric acid reduced P. acnes-induced and AA+ P. acnes-induced IL-8 expression.In addition, pretreatment with AA stimulated enhanced IL-8 by P. acnes- stimulated SZ95 sebocyte, Capric acid reduced P. acnes-induced and AA+ P. acnes-induced IL-8 expression.
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