| Summary: | 碩士 === 國立清華大學 === 生物科技研究所 === 102 === Abstract
In Drosophila, a single training session of odor-shock association can only produce labile form memory which cannot last over one day. To form aversive long-term memory (LTM), multiple training sessions and de novo protein synthesis are required. Interestingly, inhibiting de novo protein synthesis in mushroom body (MB), a well-known neuroanatomical site involved in learning and memory, does not impair LTM. Here, we ask whether there is de novo protein synthesis in MB after training, and if so, what is the function of these proteins? By specifically expressing a temperature-sensitive ribosome-inactivating toxin in MB, we found that (i) inhibition of MB protein-synthesis after training enhances three-hour memory retention score. (ii) The enhanced memory is resistant to anesthesia but insensitive to serotonin synthesis inhibition which impairs radish-dependent anesthesia-resistant memory (ARM). (iii) The enhanced memory is derived from anesthesia-sensitive memory but is able to last over one day. Based on these findings, we propose that the main function of post-training-synthesized proteins in MB is to prevent premature memory from consolidating into long-lasting form, and inhibition of MB protein-synthesis after training allows early-consolidation of labile memory into anesthesia-resistant, long-lasting memory after only one single session of training.
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