| Summary: | 碩士 === 國立中山大學 === 生物醫學研究所 === 102 === Cryptocarya-derived natural products were reported to have many biological effects such antiproliferation of some cancers. However, the possible antioral cancer effect of Cryptocarya-derived natural products was less addressed. In this study, we firstly the methanol extract of Cryptocarya plant root (MECCrt) to evaluate its potential function of antioral cancer. We found that MECCrt significantly reduced cell viability of two oral cancer Ca9-22 and CAL 27 cells in dose-responsive manners (p < 0.01). For the flow cytometry, the sub-G1 population and annexin V-intensity of MECCrt-treated Ca9-22 and CAL 27 cells significantly accumulated in a dose-responsive manner (p < 0.01). These apoptotic effects were associated with the findings that intracellular ROS generation were induced in MECCrt-treated Ca9-22 and CAL 27 cells in dose-responsive and time-dependent manners (p < 0.01). MECCrt also displayed significant reduction of mitochondrial membrane potential in these two cells in a dose-responsive manner (p < 0.01–0.05). Taken together, these results suggest that MECCrt has an antiproliferative potential against oral cancer cells through apoptosis, ROS induction, and mitochondria membrane depolarization.
|
|---|
