Phytochemical compounds and chemoprotective effect of dried Siraitia grosvenorii fruit extracts

• Main Authors: Prokprae Puwavicheanchai, 卜瑋彩
• Other Authors: Ming-Chang Wu
• Format: Others
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/42275366936480282846

碩士 === 國立屏東科技大學 === 熱帶農業暨國際合作系 === 102 === The fruits of Siraitia grosvenorii (Luo Han Kuo), belonging to family Cucurbitaceae, are traditional Chinese medicine and edible plant cultivated in south-western China. S. grosvenorii fruit extracts have been approved generally recognized as safe (GRAS) status by the Food and Drug Administration of the United States (USFDA). The effects of extraction solvents (hot water and 80% ethanol) in dried fruit flesh and peel extraction and effects of yeast strains (Saccharomyces bayanus and S. cerevisiae) and fermentation time in dried fruit flesh and peel fermentation were investigated for its antioxidant capacity and phytochemical components and hepatoprotective activity on ethanol injured mouse hepatocytes FL83B. The antioxidant activity was evaluated with 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing ability of plasma (FRAP), and reducing power assay. Quantitative and qualitative analysis of phytochemical compounds contained in extracts and fermented dried fruits were carried out by RP–HPLC revealed one triterpene glycoside (mogroside V) and six active phenolic compounds (gallic acid, caffeic acid, p-coumaric acid, catechin, rutin and quercetin). These results revealed that dried S. grosvenorii fruit peel in hot water extracts (PH) contained mogroside V, phenolic acids, flavonoids and exhibited higher than others extracts. However, fermented dried S. grosvenorii fruit peel by S. bayanus (PSB) showed the highest antioxidant activities and phenolics content compare to all samples, and also showed suppressed injured cell by alcohol. In Cytotoxicity assay, dried S. grosvenorii fruit extracts at the concentrations less than 1,000 μg/mL were considered to be non toxic to mouse hepatocytes FL83B and 500 μg/mL of dried S. grosvenorii fruit peel in hot water extract (PH) showed the most significant protective ability on ethanol injured cells. This ability may be related with antioxidant properties of dried S. grosvenorii fruit peel extract contributed by its compounds, which are mogroside V, phenolic acids and flavonoids. Hence, we proposed that dried S. grosvenorii fruit extract could potentially serve as hepatoprotective agent from natural source against alcohol-induced oxidative stress.