| Summary: | 碩士 === 國立高雄師範大學 === 生物科技系 === 102 === 英文摘要
Burkholderia pseudomallei is a gram-negative rod, causing a zoonotic infection (called melioidosis). The flagellum of B. pseudomallei is recognized as a virulent factor involving in systemic dispreading of the bacteria to remote organs from infected foci. The flagellum is cosisted of filament, hook and basal body. The basal body is located at inside of cell wall while filment can outward elongate through hollow of hook from basal body. At least, several sets of clustered genes included flagellar synthesis, relgulation, movement, chemotaxis, signal transduction, motor and proton motive force are involved in the function of bacterial movement. To explore the genetic diversities, the bioinformatics of flagella genomes were analyzed from differet representative strains of B. pseudomallei.
Nowadays the whole gemones of B. pseudomallei 1710b, 1106a, k96243 and 668 as well as an indigenous strain of B. pseudomallei vgh19 were sequenced. By the bioinformatic analysis in which based on the catagories of Kyoto Encylopedia, the nucleotide sequences of flagella assembly,type Ⅲ secretion system, chemotaxis and other anticipated genes related to flagellar function were retrieved and analyzed. All open reading frames as well as their encoding amino acid sequences and proteomic informations including general physic and chemical characteristics, orthologous homology as well as modification of individual proteins were discussed.
Our results indicated that, for genome sequences of all strains, the distinct motifs of certain genes can be annoted although the most genes were conserved. In spite of alternative initiation codons, gene overlapped and different copy numbers of flagella gene were existed in genome among comparable strains, the protein sequences and functional domains of B. pseudomallei vgh19 harbouring flagella proteins were highly conserved. We suggests that the flagella proteins of B. pspeudomallei vgh19, a unique strain in Taiwan about world, were conserved despite this strain could independently evoluted in Taiwanese environments.
Key word: Burkholderia pseudomallei, annotation, diversity, bioinfromatics
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