Expression of zebrafish camsap genes in the embryos and adult tissues

• Main Authors: Jhen-Jie Sun, 孫振傑
• Other Authors: Wen-Wen Lin
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/57acx4

碩士 === 國立高雄海洋科技大學 === 海洋生物技術研究所 === 102 === CAMSAP, calmodulin regulated spectrin-associated protein, is a microtubule minus-end binding protein. In zebrafish, there are four camsap genes, camsap1a, camsap1b, camsap2 and camsap3. Mammalian CAMSAPs stablilize and regulate the polymerization of minus-end of noncentrosomal microtubule via a conserved CKK domain in their C-termini. The expression profiles and functions of camsap genes during embryonic development are unclear. In this study, the spatiotemporal expression patterns of zebrafish camsap genes were studied. We analyzed the nucleotide and amino acid sequences of four zebrafish camsaps and found that they all contain the conserved CKK domain in their C-termini and a variable region in the middle of proteins. RT-PCR analyses of adult tissues showed that camsap1a is expressed in the brain, camsap1b expressed in the muscle and testis, camsap2 expressed in the brain, muscle, testis and eyes, and camsap3 expressed in the muscle, ovary and testis. RT-PCR analyses of embryos at various stages showed that all four camsap genes are maternally expressed and deposited in the embryos at cleavage and blastula stages. Whole-mount in situ hybridization further revealed that the transcripts of all four camsap genes are initially maternally deposited in the cleavage and blastula embryos. The zygotic expressions of camsap genes start to differentiate from gastrula to hatching stages. Though camsap1a and camsap1b are all expressed in the midbrain, hindbrain, otic vesicles, retina, pectoral fins and yolk syncytial layer, the expression of camsap1b in the yolk syncytial nuclei and gut primordium is weaker. camsap2 and camsap3 are also expressed in the midbrain, hindbrain, otic vesicles, pectoral fins, and yolk syncytial layer, but the expression of camsap3 is lower in the retina and yolk syncytial layer.