Secreted Vago restrict Dengue virus infection in Aedes aegypti mosquito by activating the Jak-STAT pathway

• Main Authors: Hsuan Pai, 白軒
• Other Authors: Chang-Chi Lin
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/75ekg9

碩士 === 國防醫學院 === 微生物及免疫學研究所 === 102 === Though dengue virus infection is a major public health problem around the word, the mechanisms of antiviral immunity in the vector mosquitoes are poorly understood. The drosophila Dicer-2 was demonstrated to activate antiviral gene, Vago, and ortholog Vago of Culex mosquito was also revealed to up-regulated in response to West Nile virus infection. In this pilot study, we demonstrated that Aedes aegypti Vago was also a secreted peptide and could restrict dengue virus infection by activation the Jak-STAT pathway. The Aedes Vago gene was cloned and expressed, and polyclonal antibody against the protein was produced. Knockdown the Vago, Dicer-2, or STAT gene in Aedes aegypti cell line and Aedes aegypti mosquito with specific siRNA then infected with dengue virus showed virus up growth, while overproduction of Vago in Aedes aegypti cell line revealed down growth of dengue virus. Real time PCR assay of Vago, Dicer-2, STAT, and dengue viral gene and Western blot assay of Vago protein were carried out in the above experimental knockdown or overexpression procedures. This study revealed that the Aedes aegypti Vago could antagonize dengue virus infection through this mosquito immune pathway.