The correlation of Meta1 gene with cancer metastasis and its effect on cancer cell inva-sion and migration

碩士 === 國防醫學院 === 生物化學研究所 === 104 === Cancer metastasis limits therapeutic options and is the leading cause of cancer mortality. Therefore, it is pivotal to decipher the underlying mechanisms involved in cancer metastasis. We found that Meta1 is highly expressed in multiple cancer cell lines with hig...

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Main Authors: Huang Wenyu, 黃文俞
Other Authors: Cha Tailung
Format: Others
Language:zh-TW
Published: 2015
Online Access:http://ndltd.ncl.edu.tw/handle/55732241513435823318
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spelling ndltd-TW-102NDMC01070012017-09-03T04:24:45Z http://ndltd.ncl.edu.tw/handle/55732241513435823318 The correlation of Meta1 gene with cancer metastasis and its effect on cancer cell inva-sion and migration Meta1基因與癌症轉移的相關性及對癌細胞移動性和侵犯性之影響 Huang Wenyu 黃文俞 碩士 國防醫學院 生物化學研究所 104 Cancer metastasis limits therapeutic options and is the leading cause of cancer mortality. Therefore, it is pivotal to decipher the underlying mechanisms involved in cancer metastasis. We found that Meta1 is highly expressed in multiple cancer cell lines with high metastasis potential. There is a positive correlation between Meta1 expression levels and metastasis potential in cancer cells. Overexpression of Meta1 results in cell morphological change with epithelial-to-mesenchymal transition (EMT). Overexpression of Meta1 leads to EMT phenotype without alteration of EMT markers. Meta1 induces cytoskeletal rearrangement of F-actin contributing to cellular morphological change. In addition, Meta1 induces filopodia formation which is correlated with Rho signaling activation. Meta1 induces EMT cellular morphological change and enhance cell migratory ability which may contribute to cancer call metastasis. We also found that DNA methylation status of Meta1 promoter region was correlated with Meta1 expression levels in multiple cancer cell lines. However, the Meta1 expression was slightly induced by DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine suggesting that epigenetic regulation was involved in Meta1 expression. Our study reveals Meta1 might play a role in the regulation of EMT-independent migtatory function contributing to cancer metastasis. Cha Tailung Huang Shihming 查岱龍 黃世明 2015 學位論文 ; thesis 76 zh-TW
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description 碩士 === 國防醫學院 === 生物化學研究所 === 104 === Cancer metastasis limits therapeutic options and is the leading cause of cancer mortality. Therefore, it is pivotal to decipher the underlying mechanisms involved in cancer metastasis. We found that Meta1 is highly expressed in multiple cancer cell lines with high metastasis potential. There is a positive correlation between Meta1 expression levels and metastasis potential in cancer cells. Overexpression of Meta1 results in cell morphological change with epithelial-to-mesenchymal transition (EMT). Overexpression of Meta1 leads to EMT phenotype without alteration of EMT markers. Meta1 induces cytoskeletal rearrangement of F-actin contributing to cellular morphological change. In addition, Meta1 induces filopodia formation which is correlated with Rho signaling activation. Meta1 induces EMT cellular morphological change and enhance cell migratory ability which may contribute to cancer call metastasis. We also found that DNA methylation status of Meta1 promoter region was correlated with Meta1 expression levels in multiple cancer cell lines. However, the Meta1 expression was slightly induced by DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine suggesting that epigenetic regulation was involved in Meta1 expression. Our study reveals Meta1 might play a role in the regulation of EMT-independent migtatory function contributing to cancer metastasis.
author2 Cha Tailung
author_facet Cha Tailung
Huang Wenyu
黃文俞
author Huang Wenyu
黃文俞
spellingShingle Huang Wenyu
黃文俞
The correlation of Meta1 gene with cancer metastasis and its effect on cancer cell inva-sion and migration
author_sort Huang Wenyu
title The correlation of Meta1 gene with cancer metastasis and its effect on cancer cell inva-sion and migration
title_short The correlation of Meta1 gene with cancer metastasis and its effect on cancer cell inva-sion and migration
title_full The correlation of Meta1 gene with cancer metastasis and its effect on cancer cell inva-sion and migration
title_fullStr The correlation of Meta1 gene with cancer metastasis and its effect on cancer cell inva-sion and migration
title_full_unstemmed The correlation of Meta1 gene with cancer metastasis and its effect on cancer cell inva-sion and migration
title_sort correlation of meta1 gene with cancer metastasis and its effect on cancer cell inva-sion and migration
publishDate 2015
url http://ndltd.ncl.edu.tw/handle/55732241513435823318
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