The correlation of Meta1 gene with cancer metastasis and its effect on cancer cell inva-sion and migration
碩士 === 國防醫學院 === 生物化學研究所 === 104 === Cancer metastasis limits therapeutic options and is the leading cause of cancer mortality. Therefore, it is pivotal to decipher the underlying mechanisms involved in cancer metastasis. We found that Meta1 is highly expressed in multiple cancer cell lines with hig...
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| Format: | Others |
| Language: | zh-TW |
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2015
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| Online Access: | http://ndltd.ncl.edu.tw/handle/55732241513435823318 |
| Summary: | 碩士 === 國防醫學院 === 生物化學研究所 === 104 === Cancer metastasis limits therapeutic options and is the leading cause of cancer mortality. Therefore, it is pivotal to decipher the underlying mechanisms involved in cancer metastasis. We found that Meta1 is highly expressed in multiple cancer cell lines with high metastasis potential. There is a positive correlation between Meta1 expression levels and metastasis potential in cancer cells. Overexpression of Meta1 results in cell morphological change with epithelial-to-mesenchymal transition (EMT). Overexpression of Meta1 leads to EMT phenotype without alteration of EMT markers. Meta1 induces cytoskeletal rearrangement of F-actin contributing to cellular morphological change. In addition, Meta1 induces filopodia formation which is correlated with Rho signaling activation. Meta1 induces EMT cellular morphological change and enhance cell migratory ability which may contribute to cancer call metastasis. We also found that DNA methylation status of Meta1 promoter region was correlated with Meta1 expression levels in multiple cancer cell lines. However, the Meta1 expression was slightly induced by DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine suggesting that epigenetic regulation was involved in Meta1 expression. Our study reveals Meta1 might play a role in the regulation of EMT-independent migtatory function contributing to cancer metastasis.
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