Trichoderma harzianum ETS 323 mediated resistance in Brassica oleracea var. capitata against Botrytis cinerea B134

• Main Authors: Chuan-Kai Liao, 廖傳凱
• Other Authors: Kou-Cheng Peng
• Format: Others
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/984j52

碩士 === 國立東華大學 === 生命科學系 === 102 === Trichoderma spp are well known biocontrol agents by the possible mechanisms including antibiotic secondary metabolites and host resistance induction. We like to learn if there is any relationship between the antibiotic secondary metabolites and the host resistance induction. Anthraquinone and chrysophanol are two Trichoderma metabolites, among others, have antibiotic activity and been isolated. A three way interaction was established to test Brassica oleracea var. capitata (cabbage) resistance induction against Botrytis cinerea by the two metabolites. Morphologically, cabbage leaves showed less infection area of each compound treated than of the Trichoderma harzianum ETS 323 treated or not treated. The proteome of each 50 ppm metabolite pretreated 24 h, 2 d B. cinerea infected, 4wks old cabbage leaves as well as T. harzianum ETS 323 5 d pretreated 4wks old cabbage leaves were investigated. Up-regulated protein spots were identified as Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), Ribulose-1,5-bisphosphate carboxylase activase, and ATP synthase in compounds treated and T. harzianum ETS 323 treated cabbage leaves.   These results suggested anthraquinone and chrysophanol may play partial role of T. harzianum ETS 323 induced cabbage resisitance against B. cinerea. The amount of cabbage mRNAs, in the presence 10 ppm of anthraquinone (A) or chrysophanol (C) determined by the q-PCR, of Rubisco (1.50/A, 1.38/C), Rubisco activase (4.03/A, 22.32/C) and ATP synthase (1.12/A, 1.04/C) along with sucrose 6- phosphate synthase (0.95/A, 1.24/C), sucrose transporter 1 (0.46/A, 1.23/C), 2 (0.82/A, 1.27/C), and 3 (1.38/A, 1.36/C) increased. This research showed the Trichoderma spp anthraquinones can promote cabbage photosynthesis to increase the product of photosynthesis glucose converted to sucrose by the increasing the amount of sucrose 6- phosphate synthase. Sucrose was further facilitated exporting and transporting by increasing the number of sucrose transporting related proteins SUT1, 2 and 3 to the root of cabbage. The sucrose in root was either secreted directly to the rhizosphere of converted to fructose and glucose by the sucrose invertase before released to the rhizosphere full of Trichoderma.