Peritoneal dopamine administration increased cell-mediated cytotoxicity of both innate and acquired anti-tumor immunity

• Main Authors: Yun-Ju Li, 李韻如
• Other Authors: Bor-Chun Weng
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/79014098640629398613

碩士 === 國立嘉義大學 === 微生物免疫與生物藥學系研究所 === 102 === Cancer has been the main cause of death in Taiwan, and personal physiology , drug overdose toxicity or other factors in the body are bring to the patient additional burden in current. Although the specific immunotherapy about antibodies and cytokines can reduce these situations, but it still with the anti-cancer drugs. Dopamine has been reported to cause the shrink of tumor cells, resulting in the ability to inhibit angiogenesis and apoptosis in tumor cells. So exploring the concentration does that is not cause normal cells damage, regulating the immune system and to find out the other kinds of ways to increase the ability of dopamine in anti-cancer. It is still awaited to explore if dopamine exhibit an immunomodulatory role in anti-cancer immunity at physiological concentration. These experiments confirm that dopamine could elevate both innate and adaptive immunity mediated cytotoxic on tumor cells at a concentration of 10 ng/mL treatment. Specific and nonspecific cell mediated cytotoxic activities were assessed cytotoxic mediators； Granzyme B, lysozyme gene expression level increased in the RBC-removed splenocytes treating dopamine in ex-vivo and in vivo settings, the splenocytes treated by dopamine release dopamine as a weapon to attack tumor cells and it also increases the expression of dopamine transporter gene expression level. Furthermore, dopamine uptake and release in resting or activating states of splenocytes were also evaluated by analysis of cell-homogenates via HPLC. Reuptake inhibitor reserpine was used. The VMATs mediated dopamine uptake in speno-lymphocytes was abolished. The killing ability of natural killer cells was also significantly decreased. Whereas in this case the ability with a significant increase in specificity when the splenocytes recognize tumor cells. In vivo experiments were found the tumor bearing mice injection dopamine with the ability of killing tumor cells in significantly increasion, and the ratio of natural kill cells in splenocytes is also increasing. Our study demonstrated the ability of dopamine can be achieved to regulate immune cells in non-specific and specific recognizion and regulate the release of dopamine path. Perhaps dopamine with auxiliary potential with anti-cancer drugs, in addition to normal cells without increasing the burden of the concentration under strengthen, but also to provide less economic pressure in anti-cancer.