Correlation of gene polymorphisms and other risk factors in canine pancreatitis

• Main Authors: Fan, Ming-Huei, 范明惠
• Other Authors: Chou, Shih-Jen
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/83xe37

碩士 === 國立嘉義大學 === 獸醫學系研究所 === 102 === Pancreatitis is the most common exocrine pancreatic disease in dogs. The etiology and pathogenesis of canine pancreatitis is not clearly understood. In human, there are many risk factors have been confirmed for pancreatitis, and the hypertriglyceridemia (HTG) is one of the important causes. Nevertheless, the influences of these factors on canine pancreatitis are undefined. We screened canine PRSS1 and SPINK1 gene in 46 dogs: 29 dogs clinically diagnosed with pancreatitis (cases) and 17 dogs without pancreatitis (controls). Furthermore, serum triglyceride (TG) concentrations are compared between two groups to study on the correlation among gene polymorphisms, HTG and canine pancreatitis. Several single nucleotide polymorphisms (SNPs) were identified in PRSS1 gene, and there is no evidence that the SNPs are correlative to pancreatitis. Two mutations were identified in SPINK1 gene: c.60C>A and c.74A>C. Two-way analysis of variance were used to analyze the influence of factors on pancreatitis, and the data showed no interaction between genotype and HTG (P > 0.1). The two SNPs could be associated (P < 0.1) with pancreatitis in dogs. The dominant, recessive, multiplicative, and additive models were carried out to explore how these two SNPs to affect the presence of canine pancreatitis. In the dominant model, the genotype distribution doesn’t differ (P > 0.1) between cases and controls. In the recessive model, the cases have significantly higher proportion of dogs with homozygous mutation (P < 0.05). In the additive model, the trend of risk increased with amount of minor allele was observed (P < 0.1). In the multiplicative models, the cases have significantly higher variant allele frequency (P < 0.05) than controls. To the authors’ knowledge, this is the first study in which variants of the SPINK1 gene was identified to be associated with pancreatitis in dogs besides Miniature Schnauzer. Furthermore, this is the first study on correlation between serum TG concentrations and pancreatitis in dogs with clinical signs besides Miniature Schnauzer. In this study, HTG seems not to be the important cause of pancreatitis. Common risk factors, like diet, overweight, and serum TG concentrations were not statistically significant between cases and controls. We only observed the trend that the higher frequency of SPINK1 gene polymorphism, the higher risk of pancreatitis in dogs.