| Summary: | 碩士 === 國立嘉義大學 === 獸醫學系研究所 === 102 === Caprine arthritis encephalitis (CAE) is an important infectious disease which can be found in goats worldwide. Clinical symptoms include arthritis, meningitis, interstitial pneumonia and mastitis. The transmission route of CAE is, mainly, by passing the pathogens through colostrum/milk from dams to lambs. There is no vaccine or effective treatment against CAE, however, the feeding of pasteurized caprine colostrum or bovine colostrum can effectively block the transmission of pathogens through the colostrum or milk to lambs. Colostrum contains immunoglobulins, lactoferrin and complement substances, which can boost immunity in lambs and are crucial for newborn animals to fight against diseases. However, the substances contained in colostrum or milk will degrade if pasteurized. Bovine colostrum is different from caprine colostrum, and while consumed by lambs, the substances which enhance the ability of neutrophils may not be absorbed and used effectively. It may lead to insufficiency in cellular components of innate immunity of lambs. This experiment will determine the phagocytic and oxidative burst ability of neutrophils obtained from lambs which were fed with various kinds of colostrum (raw goat colostrum, pasteurized caprine colostrum, pasteurized bovine colostrum and bovine colostrum made by commercial bovine colostrum powder) by flow cytometry. According to statistical analysis of the results from flow cytometry, the phagocytizing and oxidizing neutrophils channel number from lambs fed with raw caprine colostrum are significant higher than the lambs fed with pasteurized caprine colostrum in one of the goat farm. But the phagocytic ability of neutrophils from the lambs fed with pasteurized goat colostrum is significantly higher than the lambs fed with raw goat colostrum in the other goat farm. There is no significant difference in phagocytic and oxidative burst ability of neutrophils obtained from the lambs that were fed with bovine colostrum and goat colostrum.
|
|---|
