| Summary: | 碩士 === 國立中央大學 === 生命科學系 === 102 === Characterizing FoxO6 gene in mouse skeletal muscle
Forkhead box O (FoxO) transcription factor family that membrane, including FoxO1, FoxO3, FoxO4 and FoxO6 are they crucial for the regulation of metabolism, cell cycle, cell death, and cell survival. Among these FoxOs, the recently discovered FoxO6 is differs from other by lacking C-terminal PKB sites and which impairs its subcellular shuttling ability. FoxO6 is not only majorly expressed in the tissue of brain, but also expressed in the muscle. In the past, the reported that FoxO could activated Atrogen-1and MuRF1, which ubiquitin ligase expressed lead to muscle atrophy. However, the function of FoxO6 in skeletal muscle and the subcellular and fiber-type specific localization during myogenesis in vitro and in vivo are largely unknown. In addition, whether FoxO6 participates in muscle ischemia has not been reported before. We find out FoxO6 protein has higly expression in the muscle ischemia. In addition, we also discover both of the Atrogen-1 and MuRF1 are higly expression in the C2C12 FoxO6 knock down cell line. This result is obvisouly different to other FoxO members. On the other hand, we want to set up a Tet Off FoxO6 cell line system that could swithch gene on and off by tetracycline. In another aspect the size of FoxO6 is 559 and 640 amino acids that FoxO6 has reported by team of Schmidt and IMAGE clone. But the size of FoxO6 is different from them by our observating. So we guess the pass reported sequence that is not FoxO6 whole sequence. It may have another undiscover sequenc in FoxO6 5’ UTR. We successful to discover a new FoxO6 sequence with transcription start site. In the future we hope to clarify the character of FoxO6 in the muscle cell by overexpression and knock its expression.
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