| Summary: | 碩士 === 國立交通大學 === 分子醫學與生物工程研究所 === 102 === Mammalian sterile20-like protein kinase 3 (Mst3), a member of GCK-type Ste20-like protein kinase family, was reported recently to play important roles to trigger both caspase-dependent and –independent apoptotic pathways in response to staurosporine. Recently, Mst3 was shown to distribute in both cytoplasma and mitochondria. To investigate the role of Mst3 in mitochondria dynamics and morphology, we treat EBSS for 0, 2, 4, 8 h in HeLa,and HeLa (siMst3) cells. After treatment, by western blotting and immunostain observe the different between HeLa and HeLa (siMst3). The result indicated that Mst3 regulate different type of Drp1 (pS616) and Drp1 (pS637). The morphology of mitochondria between HeLa and HeLa (siMst3) were significant different, the HeLa cell of mitochondria morphology were longer and cluster in nucleus, after the starvation, the density of mitochondria became more closely. In the other hand, in the HeLa cell (siMst3) we observe the morphology of mitochondria more short than the HeLa cell. But when the starvation exceed 4 hours, the mitochondrial morphology became longer and closely. Mst3 really play an important role in regulating mitochondria dynamics/morphology.
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