Topologic and temporal control for the expression profiles of integrin heterodimers in MG63 human osteoblast-like cells

• Main Authors: Yin, Chiou-Han, 鄞秋涵
• Other Authors: Huang, Gue-Wha
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/77566278661249214412

碩士 === 國立交通大學 === 材料科學與工程學系奈米科技碩博士班 === 102 === Aiming is to investigate that how the different nanodos array and time frames influence the integrins expression and associated combination about the integrin heterodimers. Through our studies to exactly understand that how the nanoscale surfaces regulate the integrin subunits expression and the heterodimers grouping. Then, the human osteoblast-like cells were cultured on the flat, 10 nm, 50 nm, 100 nm, and 200 nm nanodots array. Cell cultured on flat, 10 nm, and 50 nm, which cell morphology were integrated, larger cell area, and better cell attachment than 100 nm, and 200 nm can be observed. Through the distribution of vinculin and actin filament is in order to investigate the nanotopogrphic modulated-cell focal adhesion and reorganization of cytoskeleton. By the results, the cell focal adhesion protein localization demonstrated the promoting cell attachment and integrated cell cytoskeleton are contributed to the nanosurfaces, flat, 10 nm, and 50 nm. In order to study integrins expression and their pairs, the integrin genes expression results exhibited that the significant genes expression of integrin has shown in 12 and 24 hours with the preferred surfaces, flat, 10 nm, and 50 nm. In the pairing of the integrin heterodimers (α and β subunits), although the high similarities has displayed on the flat and 10 nm, 50 nm possessed the different combinations. Finally, in addition to the lower expression of integrins, the pairs on the100 nm, and 200 nm were relatively simplification. In the proteins level, which demonstrated the topologic and temporal controlled- integrin pairing, and also discovered the time-delayed about the integrin proteins level, which might be associated with the mechanism of degradation and recycling of integrins. In general, flat, 10 nm, and 50 nm scales were available in researching the topologic- and temporal-modulating pairing of integrins, and suitable in investigate the mechanism of integrin heterodimers replacing. Furthermore, the integrin varieties provide us another suggestion in controlling the cell behavior. Finally, expecting this study will contribute to the researches in the implant therapies of biomedicine.