The Effects of Zoledronic Acid and Endurance Exercise on Bone Metabolism and Energy Metabolism of Ovariectomized Rats

• Main Authors: Yu-HsienChou, 周育賢
• Other Authors: Tsang-Hai Huang
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/52552771575661885587

碩士 === 國立成功大學 === 體育健康與休閒研究所 === 102 === Purpose: The purpose of this study is to investigate the effects of bisphosphonate and endurance exercise (EXE) on bone metabolism, histomorphometry and biomechanical properties in ovariectomized rats. Methods: Thirty-five-week-old Sprague Dawley (SD) rats were used for the current study. All animals were assigned to seven groups in accordance with ovariectomy (OVX)/sham surgery (SHA)、zoledronic acid (ZA) injection at low or medium doses (4 ＆ 20, μg/kg) and endurance exercise (EXE), which were Sham (n=9), OVX (n=8) , OVX+EXE (n=8), OVX+ZA4 (n=9) , OVX+ZA4 +EXE (n=9) , OVX+ZA20 (n=9) and OVX+ZA20+EXE (n=9). After 12-week interventions, all animals were euthanized. Samples of serum and bone were collected for analyses including serum marker assays, densitometry, static histomorphometry, dynamic histomorphometry and biomechanical three-point bending testing. Two-way ANOVA was used for data processing among ovariectomized groups. T-test was used for comparing the differences between the SHA group and the OVX group. Pearson’s correlation was used for analysising the relationship between serum of bone metabolism and glucose metabolism. Results: EXE rats were lower in weight, triglyceride, leptin and BMD of total femur, cortical bone and second spongiosa as comparing to non-EXE rats. On the other hand, ZA rats were lower in leptin, osteocalcin, and the mineralization degrees of second spongiosa and endocrinal bone comparing to non-injection rats. And, ZA rats were significantly higher in spongy bone volume, bone mechanical strength and total femoral BMD. Interestingly, ZA4 rats were higher in total, cortical and spongy BMD of femur when comparing to ZA20 rats. Conclusion: OVX rats treated by ZA4 had less bone loss and increased bone strength. Rats treated by ZA combined with EXE not only showed increased bone tissue but also decreased body weight and triglyceride.