Impact of sleep deprivation on multiple organ damage in mice

• Main Authors: Yu-HsuanFu, 傅宇瑄
• Other Authors: Ming-Yie Liu
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/58450009441181687366

碩士 === 國立成功大學 === 環境醫學研究所 === 102 === Sleep has important functions for every organ in the body and sleep deprivation leads to disorders that cause irrepairable damage. Although sleep deprivation can induce cognitive impairment and increase the risk of chronic illnesses, the effect of sleep deprivation on organ damage is still unclear. The aims of this study were to establish a model of sleep deprivation and to investigate organ damages induced in the model. In this study, mice were submitted to 0, 24, 48 and 72 h sleep deprivation induced by a modified multiple platform method. Blood and organ samples were collected. Organ damages were evaluated by biochemical analysis and histological examination. Malondialdehyde, nitrite, and pro-inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 levels were measured. Sleep deprivation induced heart and liver damages in a deprived time dependent manner. Cytokine and nitrite expression were changed in serum, liver, heart, lung and spleen. In conclusion, sleep deprivation might induce multiple organ damage with altered cytokines and oxidative stress.