| Summary: | 碩士 === 國立成功大學 === 環境工程學系 === 102 === This study developed a quantitative approach for analyzing the viable but nonculturable (VBNC) Escherichia coli. The approach is based on the use of quantitative polymerase chain (qPCR), propidium monoazide (PMA)-qPCR, and cell counting methods to quantify total, viable, and culturable cells of E. coli, respectively, and thus the VBNC and dead cells can be obtained according to the differences of the cells detected with the methods used. After detection optimization, the approach was used to study the distribution of VBNC E. coli associated with the physiological states and environmental factors such as temperature and salinity, as well as various surface water bodies. The results showed that E. coli greatly entered the VBNC state after the stationary phase and in accordance with temperature and salinity, with a degree of influence by temperature higher than salinity. The reservoir water is inhabited with higher fractions (83-97%) of VBNC E. coli than river water (49-67%) and domestic wastewater (29-29%). Overall, the results suggested that the developed approach is effective for analyzing VBNC cells of E.coli and can complement the current culturing methods to monitor E. coli in water environment.
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