Dynamic Study based on Surface-Enhanced Infrared Spectroscopy for Self-Assembled Monolayers Modified Process

• Main Authors: Yu-ChenChang, 張祐誠
• Other Authors: Hsien-Chang Chang
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/3jt8dn

碩士 === 國立成功大學 === 材料科學及工程學系 === 102 === With the development of biotechnology, the detection of macromolecules needs to fabricate a bio-affinity interface to capture or react with the target species. It should be noted that the fabrication of these modified layer often depended on the experience of researchers, and the characteristic of these biosensors were still trial and error. A novel technique is desired to investigate the structure and adsorbed orientation of these layers. ATR-SEIRAS has the advantages of high sensitivity, nondestructive test for biological molecules, nanoscale range of interface signals detection and high spatio-temporal resolution. In this study, The study is interested in the SAMs modification on gold surface. It made time-resolved SEIRAS to investigate the modification of 11-MUA SAMs in ethanol, the activation of EDC and NHSS in MES buffer, and covalent attachment of amide bonds in HEPES buffer, which can be clearly discussed to provide fundamental information for further biosensor application. The chemical absorption of 11-MUA SAMs completed within a few minutes. However, the regular arrangement of the hydrocarbon backbone is related to coverage, which lead to the modified time. The peak of the asymmetric stretch of COO can be used to evaluate the modified time is around 4 hours. The activation time is also done by the stretch of C=O of NHSS activate ester, which for 10 mM EDC and 2 mM NHS is about 50 minutes. Finally, The modification of histidine and histamine are also be investigated.