| Summary: | 碩士 === 國立成功大學 === 材料科學及工程學系 === 102 === In this in vivo study, surgical excise in mice skin was employed as the indication for imitating an acute wound. The subsequent wound management was taken by negative pressure, in comparison with a novel physical method - non-thermal micro-plasma technique. A certificated dressing was subsequently covered for both techniques. In the experiment, a 6-mm-diameter surgical excision wound in mice was created. Four study-groups on both sides of mice were examined, namely, (1) occlusive dressing (OD) after gas flow (OD-gf) and OD after micro-plasma treatment with five times (OD+MPT5) for one; (2) OD-only and OD after negative pressure treatment (OD+NP) for the other. The excitation state for micro-plasma was: the generation power of 13 W, the plasma composition of Ar with 0.5% N2 (by mass flow control), the working distance of 4 mm, and the treatment time of 30 sec per wound area to treat daily for 5 days. The applied negative pressure treatment was -90 mmHg. During micro-plasma treatment on the wound area, an average temperature of 37oC and high NO content were maintained. In the ex vivo experiment on the wound tissue lysates, ROS and RNS levels might increase with micro-plasma exposure time. The in vivo study result demonstrates that OD+MPT5 and OD+NP exhibit analogous wound closure efficacy, intensity of blood flow, and detection of total collagen fibers. By comparing their optical signals and histological examinations, the formation of new epithelium in OD+MPT5 or OD+NP on 6 days wound (dw) was earlier than the control groups (OD-only or OD-gf) and the formation of granulation tissue. It is hypothesized that OD+MPT5 is competent to stimulate the formation of re-epithelialization through the coverage of reactive plasma species, while OD+NP is advantageous to stimulate the formation of granulation tissue through the continuation of extrusion and fluid removal. Both techniques are proved to have a comparable efficacy to promote surgical excision wound healing in normal C57BL/6 mice.
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