| Summary: | 碩士 === 國立成功大學 === 生命科學系 === 102 === Comparative transcriptome analysis was performed to explore transcriptional response
of rice (Oryza sativa L. cv. TN-67) to a variety of environmental stresses. Global response
of rice to eight stress conditions: arsenate, copper, cadmium, mercury, chromate, vanadate,
ferulic acid and juglone, were analyzed using DNA microarrays. There were 539 genes
defined as general stress induced response (GSIR) genes as being induced at least two fold
change under all stress conditions. A total of 61 transcription factors (TFs) were found in
GSIR genes, and most of their Arabidopsis homologs were also generally induced under
different stresses, indicating functional conservation of these TFs in stress signaling. Three
central aspects in the early response of rice to environmental stress were discovered after
analyzing GSIR genes. These aspects includes 1) biosynthesis of ethylene, jasmonate acid
and abscisic acid as well as deactivation of gibberellin, indole-3-acetic acid and cytokinin;
2) alternation in energy metabolism such as biosynthesis of γ-aminobutyric acid (GABA); 3)
biosynthesis of lignin and suberin in the root. In addition, rice exhibited similar
transcriptional response to arsenate and juglone, especially in genes related to xenobiotic
metabolism. Genes that were specifically induced by single stress in our data, might hold
potential to be developed as a biomarker for specific environment contamination. In this
study, we discovered a candidate biomarker to detect cadmium contamination in the
environment. Our results provide insights into understanding of molecular response of rice
to environmental stresses and also demonstrated the potential of using comparative
transcriptome analysis to discover new biomarkers.
|
|---|
