Metagenomics of deserted salterns under ecological restoration

• Main Authors: Hao-ChuWang, 王顥筑
• Other Authors: Tzen-Yuh Chiang
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/v2cbwb

碩士 === 國立成功大學 === 生命科學系 === 102 === In this study, the salterns of Sutzao, Tainan were chosen for research. Saltern is a place for salt making, and it used to be a prosperous industry in Sutzao. These abandoned areas have now been transformed into a protected area by the government for educational, research, and sightseeing purposes. In previous studies have indicated that ecological restoration is highly related to plants, because their roots can eliminate and confine harmful chemicals in the soil. In this paper, we strived to find important genera related to or even indicators of environmental change in the abandoned saltern. The following are the approaches implemented to understand the saltern’s microbial community and their changing pattern: Illumina sequencing machine for next generation sequencing (NGS), PERL, and MEtaGenome Analyzer version4.70.4, R project for clustering dendrogram. The results show the taxonomy phylum Acidobacteria、Actinobacteria、Gemmatimonadetes show an increasing pattern by succession. On the other hand, Spirochaetes displayed a decreasing pattern. In this research, we found that several genera play an important role in the ecological system. Zoogloea is used in waste water treatment for its ability to lower biological oxygen demand and promote the formation of sludge deposits. Marinobacter grows at NaCl concentrations of 0.08 to 3.5 M and uses various hydrocarbons as the sole source of carbon and energy. Burkholderia, Nitrosospira are common bacteria in soil or rhizosphere, and are associated with carbon and nitrogen cycle, respectively. Still, further research is needed, there are still many beneficial genera, but how they affect local ecology remains unclear. However, some of them show the trend of saltern's succession. These genera may be used as an alternative indicator of high salt adversity to monitor microbial communities’ changes resulting from human or natural disturbances.