1. The constituents from the root of Magnolia officinalis Rehd. Et Wils.2.The constituents from the Cynanchum komarovii Al. Iljinski. and synthesis of cynankomarine A.

• Main Authors: Hung-ChengShih, 施宏誠
• Other Authors: Tian-Shung Wu
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/7d9x5r

博士 === 國立成功大學 === 化學系 === 102 === Twenty-four compounds were isolated from the ethanol extracts of roots of Magnolia officinalis Rehd. et Wils and were characterized by comprehensive analyses of their 1D and 2D NMR and mass spectroscopic data. Among these isolates, two honokilol dimers (houpulins A-B), seven neolignan derivatives (houpulins E-K) and four magnolol derivatives (houpulins C-D, L and M), which all possessed new carbon skeletons, were reported from the natural sources for the first time. In addition, all the pure compounds were evaluated for the inhibition of superoxide anion generation and elastase release and most principles exhibited significant inhibition effects. Among the tested compounds, houpulin B displayed significant inhibitory activity towards superoxide anion generation and elastase release with IC50 values of 2.85±0.16 and 2.00±0.50µM, respectively. These results indicated that these compounds should be the anti-inflammatory principles in the roots of M. officinalis. Forty-eight known compounds and eight new compounds were isolated from the methanol extracts of Cynanchum komarovii Al. Iljinski. Incuding three phenanthroindolizdine alkaloids with new carbon skeletons, namely cynankomarines A-C, and five new compounds namely cynankoarins A-E were reported from the natural sources for the first time. Cynankomarine A was belonged to the phenanthroindolizdine type alkaloid, which had drawn researchers’ attentions as they displayed potential therapeutic effects especially their profound cytotoxic activity. To evaluate its potential of cytotoxicity, the present study was aimed to the total synthesis of cynankomarine A by chemical methods to afford large quantity of samples for the further bioactivity examinations.