Proteomic Analysis of Galactosemic Rat Red Blood Cell Proteins

• Main Authors: Min-ChiTu, 杜敏綺
• Other Authors: Fu-Yung Huang
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/83718128372620118658

碩士 === 國立成功大學 === 化學系 === 102 === In this study, we used 2D electrophoresis followed by LC-MS/MS identification to screen the red blood proteins of galactosemic rats. The galactosemic rats are fed diet containing 50% galactose. After three weeks of galactose diets, the rats lens started to show cortical nucleus cataract. It was then further to study whether there is any correlation between the red blood protein and the changes of lens crystallins. In the 2D gel electrophoresis study, there was no obvious sign that red blood proteins changed with the fed time of galactose diets. But based on the MS/MS analysis data, it was found that the extent of the glycation increased with the lens morphological change of becoming progressive opaque. And it was also found advanced glycation end products (AGEs) of Carboxymethyllysine (CML). The blood test showed lower blood glucose content for experimental group compared to control group. Other test such Glutamate oxaloacetate transaminase (GOT), Glutamate pyruvate transaminase (GPT), Triacylglyceride (TG), Creatinine (Crea), Serum uric acid( SUA), Blood urea nitrogen (BUN), Total cholesterol (T-chol), Sodium ion, and Potassium ion, the concentrations were higher for experimental rat than for control one. These results indicated that under high does galactose the metabolism malfunctioned leading to the formation of cataract in a short time feeding period of three weeks.