| Summary: | 碩士 === 國立成功大學 === 化學工程學系 === 102 === In this study, a new technique which was called molecularly imprinted technique (MIT) might replace the traditional technique, which was energy and time consuming, with low cost and simple process. Genistin (Gi) was chosen as template to prepare the molecularly imprinted polymer (MIP) by precipitation polymerization. The batch rebinding method is applied to investigate the performance of the molecularly imprinted polymer in various syn-thesis conditions. When Ethylene glycol dimethacrylate (EGDMA) and 2-Dimethylamino ethyl methacrylate (DMAEMA) were used for cross-linker and functional monomer, respec-tively, there is a maximum average imprinting factor as an increase in the molar ratio of functional monomer to cross-linker, the molar ratio of template molecule and functional monomer, or concentration of template. The average imprinting factor of the polymer fabri-cated in organic solution is larger than the polymer fabricated in aqueous solution. The op-timum molar ratio of functional monomer to cross-linker, the molar ratio of template mole-cule and functional monomer, and template concentration were 3:7, 1:8 and 3.13 mM, re-spectively, in acetonitrile solution. The results showed the highest imprinting factor and av-erage imprinting factor were 2.4, and 2.0, respectively. The highest specific selectivity and separation factor among all genistin imprinted polymer were 1.7 and 4.2, respectively.When the genistin imprinted polymer was used as the packing material for the pack column in chromatography separation, the retention time for genistin and daidzin were about 15 and 2 hours, respectively, with soybean extract. The performance of synthesized genistin imprinted polymer showed a good potential in commercial application.
|
|---|
