| Summary: | 碩士 === 國立成功大學 === 化學工程學系 === 102 === Creatinine, the metabolite in muscle from creatine, is an important marker for the diagnosis of kidney function. Therefore, human blood and urine creatinine levels are the very important physiological test items. In this study, the functional monomer, 2-amino-3-nitro-5-vinylpyridine (ANVP) was synthesized to assist the production of the creatinine-imprinted polymer film creatinine sensing by AC impedance. ANVP as the monomer, ethylene glycol dimethacrylate (EGDMA) as the crosslinker, and 2, 2′-azobisisobutyronitrile (AIBN) as the initiator, were mixed together in the presence of creatinine as the template for the molecular imprinted polymeric film polymerization on the surface of Au electrode. After the template extracted, the MIP film have the ability to recognize the creatinine by its specific cavity. The most appropriate synthesized ratio is 1:3:24, and the volume of pre-polymerization solution is 7.0 μL. The sensitivity of MIP film electrode is 0.74 ± 0.03 ((Ω – Ωo)/ Ωo)/ (mg/dL), and the imprinting factor is 2.94 ± 0.18. The recognizable of MIP film electrode in the coexistence of creatinine and creatine solution would be confirmed in this study. The selectivity of MIP film electrode toward creatinine is 6.42 ± 0.62. Whether it has the function toward creatinine sensing in the serum based environment was also confirmed.
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