The Haemagglutinin of Avibacterium paragallinarum is a Trimeric Adhesin that Confers Cell Adherence and Biofilm Formation Activities
碩士 === 國立中興大學 === 微生物暨公共衛生學研究所 === 102 === The haemagglutinin (HA) protein plays a key role in the immunogenicity and pathogenicity of Av. paragallinarum. In previous studies, a 210 kDa protein (HMTp210) was reported to be the HA of Av. paragallinarum but the biological function of this HMTp210 is n...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2014
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| Online Access: | http://ndltd.ncl.edu.tw/handle/77905968329436439098 |
| Summary: | 碩士 === 國立中興大學 === 微生物暨公共衛生學研究所 === 102 === The haemagglutinin (HA) protein plays a key role in the immunogenicity and pathogenicity of Av. paragallinarum. In previous studies, a 210 kDa protein (HMTp210) was reported to be the HA of Av. paragallinarum but the biological function of this HMTp210 is not well-defined. In this study, mutant strains that lacked the HMTp210 protein were constructed by using the Targetron gene knockout system. Haemagglutination and haemagglutination-inhibition (HI) assays showed that the HMTp210-deficient mutants completely lost their haemagglutination activity and failed to elicit HI antibodies in immunized chickens. Additionally, the HMTp210-deficient mutants had a reduced ability to adhere to HeLa cells and form biofilms on abiotic surfaces. These results indicated that the HMTp210 protein is the major haemagglutination antigen of Av. paragallinarum and this protein is a trimeric autotransporter adhesin that confers cell adherence and biofilm formation activities. These results could be valuable for understanding the function of the HA and mechanism of pathogenicity of Av. paragallinarum.
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