| Summary: | 碩士 === 國立中興大學 === 奈米科學研究所 === 102 === This study started with different concentrations and particle sizes of gold. The gold nanoparticle concentrations were added into NIH 3T3 cells to observe cell growth curve. The cells of a smaller diameter of gold nanoparticles had a more significant impact, so the smaller particles sizes were selected.
The gold nanoparticles that were added into NIH 3T3 cells were found to have more swelling and dendritic became shorting observed by Optical microscope. The NIH 3T3 cells in the liquid were used to measure with the AFM (Atomic force microscope), observed the topography of NIH 3T3 cells with the nanoparticles at different times.
The second part of this study is by fluorescence microscope, the fluorescence observation have actin skeleton of cells and the endoplasmic reticulum of cells. After adding gold nanoparticle to the actin filaments of NIH 3T3 are broken and appeared as an aspiration of dots and endoplasmic reticulum of cells becomes incomplete.
The third part of the mechanical measurements is by AFM. After adding gold nanoparticles, the nucleus and dendritic adhesion force of cell became bigger than the
control with increased time. The adhesion force of nucleus is greater than dendritic.
The last part, observed the substrate after the conductor layer produced by optical microscope. The SEM (Scanning electrical microscopy) measured the thickness of the parameters of the deposition of silicon nitride insulating layer made and observed the surface. PDMS made for using cultured cells, after on the substrate
to test cells can be successfully cultured on the substrate.
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