Molecula characcterizatiion of hemoplasmas coommonllyfoundd in dogs and caats in Taaiwan

• Main Authors: SShu-Yi Chiu, 邱姝怡
• Other Authors: 鍾楊聰
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/89856293818939282952

碩士 === 國立中興大學 === 獸醫學系暨研究所 === 102 === Hemotropic mycoplassmas (hemooplasmas) are bacteriaa that infecct erythrocyytes, attaching to the red blood cell. Cats can be nfected by a range of hemoplasmmas: Mycooplasma haaemofelis Mhf), Candiidatus Mycooplasma haeemominutum (CMhm) and Canddidatus Myycoplasma turicensis (CMtc). Doogs can be infected by Mycoplaasma haemmocanis Mhc) and Caandidatus Mycoplasma haematoprravum (CMMhp). A tota of 110 dogs and 98 cats that had blood saamples submitted to eterinary aboratories were tested by a polyymerase chaain reaction (PCR) assaay which targeted the 16S rRNA gene or 166S-23S ITS region of haemoplasm species. The resultts showed that two ovine hemooplasmas, Mycoplasma wenyonii (Mw) and Candidatu Mycoplaasma haemoobos (CMMbo), were detected oth in the dogs and cats, as weell as Mhc and Mhf were deteccted respecctively. Mw and CMbbo had beeen first repported in 1934 and 2008, respeectively. Mw and CMb were nevver detected in dogs or cats, whiile CMbo had a posittive PCR reesult in a doog in Austraalia. In ordeer to make a definite ddiagnosis off the hemooplasmas foound in this study effificiently andd precisely,, we establiished a reliiable PCRR-RFLP assaay to distinnguish them easily. Phhylogenetic analysis of 16S-23S ITS regioon sequencee showed thhat CMbo hhas at least two clades.. The isolattes in this study are included in one of the clade. In conclussion, we dentified annd molecullarly charaacterized heemoplasmass in dogs annd cats in Taaiwan.