The Morphological Effects of Sensorimotor Cortex in Focal Experimental Intracerebral Hemorrhage

• Main Authors: Shao-Han Chang, 張劭涵
• Other Authors: 陳建榮
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/19876905082742780840

碩士 === 國立中興大學 === 獸醫學系暨研究所 === 102 === Cerebral stroke is the second leading cause of death worldwide. Intracerebral hemorrhage (ICH), one of the most lethal stroke subtypes, often causes neurological impairment and high mortality. At present, there were many studies about mechanisms of striatal ICH, but still a lot of need to be clarified. However, the plasticity of the cortical pyramidal cells after stroke was explored at ischemic stroke rather than ICH. In this regard, male Sprague-Dawley rats (300-550 g) were subjected to ICH induction by injection of 0.4 μL collagenase type IV-S (0.125 U/μL) into the primary somatosensory cortex of the left hemisphere, and to explore the astrocyte, microglia, nNOS and the dendritic structures of the pyramidal cell at the peri-lesion areas, including the adjacent and distant areas (M1 and M2 areas are 250–500 ?m and 1000–1250 ?m from the center of infarct), for further examination. The densities of CD11b, GFAP and nNOS immunoreactivities in the adjacent peri-infarct area were significantly increased at day 7. The densities of GFAP and nNOS immunoreactivities still increased at day 14, but the CD11b+ microglia decreased. Dendritic spines on layer V pyramidal neurons of the M1 cortex were significantly reduced at day 7 and recovered at day 14. Dendritic spines on layer V pyramidal neurons of the M2 cortex were continuously decreased until day 14. Animals showed poor motor activities at day 1, and continued to recover toward day 7 and 14 by behavioral tests after ICH induction. In conclusion, we demonstrated that the peri-infarct areas were affected more toward the center of the lesion. The behavioral performance after ICH related to the spine density of the layer V pyramidal neurons of sensorimotor cortex.